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Identification and Characterization of Human Autoreactive T Cells for Autoimmune Research
Lenka Súkeníková1, Daniela Latorre2,3
1Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2025
Summary
This chapter details methods for isolating and profiling autoreactive T cells in autoimmune diseases. These techniques enable comprehensive analysis of T cells targeting self-antigens in human samples.
Area of Science:
- Immunology
- Autoimmunity Research
- Cellular Biology
Background:
- Self-reactive T cells are central to autoimmune disorders.
- Characterizing these T cells in humans is complex.
- Understanding autoreactive T cells is crucial for autoimmune disease research.
Purpose of the Study:
- To present robust methods for isolating and profiling autoreactive T cells from human samples.
- To provide tools for systematic analysis of T cells in autoimmune diseases.
- To facilitate deeper research into the mechanisms of human autoimmune disorders.
Main Methods:
- In vitro T cell screening of human biological samples (blood, CSF, biopsies).
- Isolation of autoreactive T cells and generation of single T cell clones.
- In-depth analysis of T cell receptor (TCR) repertoire, HLA restriction, cytokine profiles, and self-epitopes.
Main Results:
- Established protocols for isolating and characterizing autoreactive T cells.
- Demonstrated feasibility of comprehensive analysis from diverse human tissues.
- Enabled detailed profiling of T cell responses against specific self-antigens.
Conclusions:
- The presented methods offer powerful tools for studying human autoimmune diseases.
- Systematic analysis of autoreactive T cells is now more accessible.
- These protocols will advance our understanding of T cell roles in autoimmunity.
Keywords:
AutoimmunityAutoreactive T cellsEpitope specificitySingle T cell cloneT cell receptor (TCR)More Related Videos
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