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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: May 1, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

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Published on: December 16, 2014

An Optimization Method of PBMC Staining and Visualization Using Fluorescence Microscopy.

Maja Sochocka¹, Aleksandra Korzeniowska¹, Klaudia Ciesielska-Figlon¹

¹Department of Physiopathology, Faculty of Medicine, Medical University of Gdansk, ul. M. Skłodowskiej-Curie 3a, 80-210 Gdansk, Poland.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|November 21, 2025

Summary

This summary is machine-generated.

This study optimizes fluorescence microscopy (FM) staining and imaging for peripheral blood mononuclear cells (PBMC). Developing a standard protocol enhances FM

Keywords:

PBMC fluorescent microscopy fluorescent staining fluorophores staining optimization standardization suspension cell culture

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Area of Science:

Immunology
Cell Biology
Microscopy

Background:

Fluorescence microscopy (FM) is vital for studying cellular processes.
Standardized protocols for peripheral blood mononuclear cells (PBMC) using FM are lacking.
Current FM techniques for PBMC present challenges in speed and reproducibility.

Purpose of the Study:

To optimize FM staining and imaging protocols for PBMCs.
To establish a standardized methodology for reliable and reproducible PBMC analysis.
To enhance the signal-to-noise ratio for high-resolution PBMC imaging.

Main Methods:

Systematic optimization of PBMC staining and FM imaging steps.
Selection of optimal fluorescent dyes.
Refinement of fixation, permeabilization, and imaging parameters.

Main Results:

Improved signal-to-noise ratio in FM imaging of PBMCs.
Development of optimized protocols for high-resolution PBMC visualization.
Establishment of a foundation for standardized FM analysis of immune cells.

Conclusions:

Optimized FM protocols can yield high-quality PBMC images.
A standardized methodology will improve the usability of FM for PBMC research.
This work advances FM applications in understanding immune cell function.