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Updated: Jun 24, 2026
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
Published on: January 12, 2013
Dipeptidyl Peptidase IV Activated Near-Infrared Fluorescent Probe for Visually Evaluating Diabetes Models under
Xiao Sun1,2, Yan Huang1, Lili Fu3,4
1School of Pharmacy, Binzhou Medical University, Yantai 264003, China.
Abstract:
The health of patients with type 2 diabetes mellitus (T2DM) may be adversely affected by microplastics (MPs) that are present in the environment. However, most toxicity studies of MPs are conducted on healthy models, which may not accurately reflect the exposure risks for diabetic individuals. Dipeptidyl peptidase IV (DPP4/CD26) is a multifunctional transmembrane glycoprotein with a broad spectrum of biological functions, and its abnormal activity is intimately associated with the development of T2DM. Therefore, noninvasive detection techniques for DPP4 have wide-ranging applications in disease diagnosis and MPs toxicity research. Here, we developed an enzyme-activatable near-infrared fluorescent probe, Cy-DPP, to monitor DPP4 activity in cells and mice exposed to MPs. Using this probe, we detected changes in DPP4 levels in healthy model mice and T2DM mouse models after exposure to MPs, respectively. Our results revealed a differential upregulation of DPP4, with varying degrees of increase observed between T2DM cells and mouse models. This study not only offers a new approach for the early diagnosis of T2DM but also highlights the significant impact of MPs on the diabetic condition.
Insights
Microplastics (MPs) may harm type 2 diabetes mellitus (T2DM) patients. A new fluorescent probe, Cy-DPP, detected increased DPP4 levels in T2DM models exposed to MPs, aiding diagnosis and MP toxicity research.
Area of Science:
- Environmental Health
- Biochemistry
- Medical Diagnostics
Background:
- Microplastics (MPs) pose environmental health risks, but their impact on type 2 diabetes mellitus (T2DM) is understudied in relevant models.
- Dipeptidyl peptidase IV (DPP4/CD26) activity is linked to T2DM development, making it a target for diagnosis and toxicity studies.
Purpose of the Study:
- To develop a noninvasive method for monitoring DPP4 activity in microplastic-exposed models.
- To investigate the differential effects of microplastics on DPP4 levels in healthy versus T2DM individuals.
Main Methods:
- Development of an enzyme-activatable near-infrared fluorescent probe (Cy-DPP).
- Application of Cy-DPP to detect DPP4 activity in microplastic-exposed cells and mouse models (healthy and T2DM).
Main Results:
- The Cy-DPP probe successfully monitored DPP4 activity in response to microplastic exposure.
- Differential upregulation of DPP4 was observed in T2DM cells and mouse models compared to healthy controls after microplastic exposure.
Conclusions:
- The study introduces Cy-DPP as a novel tool for early T2DM diagnosis and microplastic toxicity assessment.
- Microplastics significantly impact DPP4 levels, particularly in type 2 diabetes mellitus contexts, highlighting potential health risks for diabetic populations.
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