Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

metilene<sup>3</sup>: identifying DMRs across multiple conditions with auto-classification.

Nature communications·2026
Same author

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
Same author

DNAviWEB: sequencing-free clinical screening of liquid biopsies.

Nucleic acids research·2026
Same author

Comprehensive Lineage Tracing Maps the Landscape of Cell Fate Decisions in Mouse Embryogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Chronic lymphocytic leukemia with <i>IGH</i>::<i>BCL3</i>-translocation is characterized by a homogeneous and distinct genetic and epigenetic landscape.

HemaSphere·2026
Same author

The spectrum of immunoglobulin heavy chain enhancer hijacking in chronic lymphocytic leukemia.

Leukemia·2026

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Advancing Hepatocyte Purification from Human Induced Pluripotent Stem Cells for Regenerative Medicine
08:51

Author Spotlight: Advancing Hepatocyte Purification from Human Induced Pluripotent Stem Cells for Regenerative Medicine

Published on: December 1, 2023

878

Single-cell activation screen identifies hepatic maturation regulators with zonal resolution.

Atsuhiro Taguchi1, Alexandre P Magalhães1, Adriano Bolondi2

  • 1Max Planck Institute for Molecular Genetics, Berlin 14195, Germany.

Developmental Cell
|November 21, 2025
PubMed
Summary
This summary is machine-generated.

Researchers identified key transcription factors Nr1i3 and Nfix that control liver cell maturation. This discovery aids in understanding organ development and improving lab-grown hepatocytes.

Keywords:
CRISPR-screeningCypNfixNr1i3developmentliverliver zonationmaturationsingle celltranscription factor

More Related Videos

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
08:52

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

11.6K
Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
07:09

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

5.3K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Advancing Hepatocyte Purification from Human Induced Pluripotent Stem Cells for Regenerative Medicine
08:51

Author Spotlight: Advancing Hepatocyte Purification from Human Induced Pluripotent Stem Cells for Regenerative Medicine

Published on: December 1, 2023

878
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
08:52

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

11.6K
Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
07:09

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

5.3K

Area of Science:

  • Developmental biology
  • Hepatology
  • Gene regulation

Background:

  • Embryonic hepatocyte maturation involves complex gene regulatory network (GRN) rewiring.
  • Achieving fully mature, functional hepatocytes in vitro remains a significant challenge.
  • Key transcriptional regulators of late-stage liver development are not well-defined.

Purpose of the Study:

  • To identify novel transcriptional regulators governing late embryonic hepatocyte maturation.
  • To understand the mechanisms of GRN rewiring during liver development.
  • To enhance the functionality of in vitro-derived hepatocytes.

Main Methods:

  • Generation of a developmental reference atlas for mouse embryonic hepatocytes.
  • Utilized a dCas9 activation screen coupled with single-cell transcriptomics.
  • Ranked the effects of late-onset transcription regulators on hepatocyte gene expression.

Main Results:

  • Identified Nr1i3 as a potent inducer of pericentral metabolic genes.
  • Identified Nfix as a critical suppressor of embryonic and periportal gene signatures.
  • Supplementing liver zonation signals with Nr1i3 and Nfix enhanced pericentral metabolic gene expression.

Conclusions:

  • Nr1i3 and Nfix play crucial roles in orchestrating hepatocyte maturation and zonation.
  • Microenvironment-targeted approaches using identified regulators can improve in vitro cell function.
  • The study provides insights into organ maturation regulatory mechanisms and strategies for cell therapy.