Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IgA Nephropathy Across the Age Spectrum: Urgent Need to Accelerate Pediatric Access to Therapeutic Innovation.

Clinical journal of the American Society of Nephrology : CJASN·2026

Same author

The Banff 2024 Kidney Meeting Report: Rejection as a spectrum of phenotypes and focus on differential diagnostic reasoning.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026

Same author

Role of lupus nephritis classification systems in everyday clinical practice: a questionnaire-based survey of the Renal Pathology Society (RPS).

Clinical kidney journal·2026

Same author

The Oxford Classification Should Remain the Gold Standard for Classification of IgA Nephropathy: Commentary.

Kidney360·2026

Same author

A Pathway to High Quality Clinical Trials in IgA Vasculitis Nephritis: Meeting Proceedings From a Multiprofessional International Collaborative Workshop.

Kidney international reports·2026

Same author

Monoclonal Immunotactoid Glomerulopathy Associated With Chronic Lymphocytic Leukemia: A Case Report.

Cureus·2025

Search research articles

Related Experiment Video

Updated: Jan 10, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Seeing the light (chains): a paradigm shift in PGNMID.

Jean Hou¹, Mark Haas¹

¹Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California, USA.

Kidney International

|November 21, 2025

Summary

This summary is machine-generated.

Most cases of proliferative glomerulonephritis with monoclonal Ig deposits lack true monoclonality, challenging their classification and treatment. New evidence suggests these kidney disorders may not stem from a single abnormal plasma cell clone.

More Related Videos

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Related Experiment Videos

Last Updated: Jan 10, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Area of Science:

Nephrology
Immunology
Pathology

Background:

Proliferative glomerulonephritis with monoclonal Ig deposits (PGMID) was historically defined by glomerular deposits of single immunoglobulin (Ig) isotypes.
The monoclonality of these Ig deposits has been questioned due to difficulties in detecting monoclonal paraproteins.

Purpose of the Study:

To re-evaluate the monoclonality of immunoglobulin deposits in proliferative glomerulonephritis with monoclonal Ig deposits.
To assess the implications of deposit monoclonality on the classification of PGIMD as a monoclonal gammopathy of renal significance (MGRS).

Main Methods:

Utilized highly sensitive and specific methods to analyze immunoglobulin deposits in kidney biopsies.
Investigated the presence and clonality of immunoglobulin proteins within the glomeruli.

Main Results:

Provided strong evidence against true monoclonality in the majority of proliferative glomerulonephritis with monoclonal Ig deposits cases.
Demonstrated that immunoglobulin deposits in these kidney diseases are often polyclonal or show ambiguous clonality.

Conclusions:

Findings challenge the current definition and classification of proliferative glomerulonephritis with monoclonal Ig deposits.
The results suggest that PGIMD may not always represent a monoclonal gammopathy of renal significance, potentially altering therapeutic strategies.