Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programming Brain Cell-Type-Selective Delivery In Vivo with Transporter-Guided Therapeutics.

bioRxiv : the preprint server for biology·2026
Same author

Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.

Neurophotonics·2026
Same author

Prodromal changes in cortical neuron physiology before amyloid pathology in a mild model of Alzheimer's disease.

Scientific reports·2026
Same author

Stochasticity in action potential backpropagation: consequences for neuronal computation.

Frontiers in cellular neuroscience·2026
Same author

Microglia Rank signaling regulates GnRH neuronal function and the hypothalamic-pituitary-gonadal axis.

Science (New York, N.Y.)·2026
Same author

Corrigendum to "Accumulation of neutral lipids in dystrophic neurites surrounding amyloid plaques in Alzheimer's disease" [2024 Apr;1870(4):167086.].

Biochimica et biophysica acta. Molecular basis of disease·2026

Related Experiment Video

Updated: Jan 6, 2026

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

31.9K

BACE1 Expression Is Required for Proper Synaptic Vesicle Dynamics in the Hippocampus.

John Zhou1, Srdjan D Antic1, Brati Das1

  • 1Department of Neuroscience, UConn Health, Farmington, Connecticut, USA.

Journal of Neurochemistry
|November 22, 2025
PubMed
Summary

BACE1 inhibition impairs synaptic release and downregulates key synapse proteins, suggesting it may hinder Alzheimer's disease treatment by affecting neuronal function.

Keywords:
BACE1synaptic plasticitysynaptic vesiclessynapto‐pHluorintranscriptomic analysis

More Related Videos

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
10:03

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Published on: August 10, 2014

12.5K
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

10.4K

Related Experiment Videos

Last Updated: Jan 6, 2026

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

31.9K
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
10:03

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Published on: August 10, 2014

12.5K
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

10.4K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Beta-secretase 1 (BACE1) cleaves amyloid precursor protein, producing β-amyloid peptides central to Alzheimer's disease (AD).
  • BACE1 inhibition is a therapeutic strategy for AD, but clinical trials show limited efficacy.
  • Previous studies indicate BACE1 inhibition impairs synaptic strength and density.

Purpose of the Study:

  • To investigate the impact of BACE1 inhibition on synaptic vesicle exocytosis and endocytosis.
  • To explore the molecular mechanisms underlying synaptic deficits caused by BACE1 deficiency.

Main Methods:

  • Utilized a synapto-pHluorin mouse model to study activity-dependent synaptic vesicle dynamics.
  • Performed transcriptomic analysis to identify gene expression changes in BACE1-deficient mice.
  • Conducted pathway analysis to elucidate affected molecular signaling cascades.

Main Results:

  • BACE1-deficient mice exhibit impaired synaptic release.
  • Transcriptomic analysis revealed significant downregulation of genes involved in synapse structure and function.
  • Pathway analysis indicated downregulation of the neurexin-neuroligin pathway, crucial for synaptic vesicle release.

Conclusions:

  • BACE1 deficiency leads to deficits in synaptic vesicle exocytosis.
  • Downregulation of key synaptic proteins, including those in the neurexin-neuroligin pathway, contributes to these deficits.
  • These findings suggest potential mechanisms for the lack of efficacy in BACE1 inhibitor-based Alzheimer's disease therapies.