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The Nutrigenomic Effect of Mela Rosa Marchigiana Callus Extract on Cellular Senescence: Insight From a Preliminary In
Chiara Rucci1,2, Enrica Sordini3, Giuseppe Persico4
1School of Advanced Studies, University of Camerino, Camerino, MC, Italy.
Molecular Nutrition & Food Research
|November 22, 2025
Summary
Mela Rosa Marchigiana callus extract (MRME) shows anti-aging and anti-inflammatory effects by downregulating TNF-α signaling in senescent cells. These benefits occur independently of DNA methylation changes, suggesting potential for further research.
Area of Science:
- Biochemistry
- Cell Biology
- Genomics
Background:
- Cellular senescence contributes to aging and inflammation (inflammaging).
- Mela Rosa Marchigiana (MRM) apple variety yields callus extracts with potential therapeutic properties.
- Pentacyclic triterpenic acids in MRM callus extract (MRME) suggest anti-inflammatory and anti-aging roles.
Purpose of the Study:
- To investigate the transcriptomic and epigenetic effects of MRME on cellular senescence.
- To evaluate MRME's impact on senescent human umbilical vein endothelial cells (sHUVECs).
Main Methods:
- In vitro cell culture of young (yHUVECs) and senescent (sHUVECs) HUVECs.
- Treatment of sHUVECs with MRME.
- Transcriptomic analysis (RNA sequencing) to compare gene expression profiles.
- Genome-wide DNA methylation analysis using Oxford-Nanopore sequencing.
Main Results:
- Cellular senescence significantly alters HUVEC transcriptome.
- MRME downregulates TNF-α signaling genes in sHUVECs, normalizing expression towards yHUVEC levels.
- MRME treatment did not induce significant changes in genome-wide DNA methylation.
Conclusions:
- MRME exhibits anti-inflammatory and anti-aging effects in vitro.
- The mechanism appears to involve modulation of inflammaging-related gene expression, independent of DNA methylation.
- MRME is a promising candidate for further in vivo studies on inflammaging.
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