Two-Dimensional LC-MS/MS Determination of Chiral Amino Acids in Real-World Samples

Area of Science:

• Biochemistry
• Analytical Chemistry
• Biomarker Discovery

Background:

• D-amino acids are emerging as significant physiologically active substances and biomarkers.
• Their presence in biological samples is often at trace levels, necessitating highly sensitive analytical techniques.
• Chiral amino acid analysis is gaining importance in various scientific fields.

Purpose of the Study:

• To summarize the design and development of multidimensional liquid chromatography (LC) systems.
• To highlight the application of two-dimensional LC-tandem mass spectrometry (2D-LC-MS/MS) for chiral amino acid determination.
• To review methods for analyzing D-amino acids in complex biological and fermented matrices.

Main Methods:

• Development and application of multidimensional liquid chromatography (LC) systems.
• Utilization of two-dimensional LC coupled with tandem mass spectrometry (2D-LC-MS/MS).
• Analysis of chiral amino acids in diverse real-world samples.

Main Results:

• Successful application of 2D-LC-MS/MS for sensitive and selective determination of trace D-amino acids.
• Demonstration of the methods' efficacy across various matrices including urine, plasma, fermented products, and proteins.
• Validation of advanced LC systems for complex chiral analyses.

Conclusions:

• Multidimensional LC, particularly 2D-LC-MS/MS, provides essential sensitive and selective methods for D-amino acid determination.
• These analytical advancements are critical for utilizing D-amino acids as biomarkers and understanding their physiological roles.
• The described methods are applicable to a wide range of real-world samples, facilitating further research.