Related Experiment Video
Updated: Jan 10, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Applications of Chiral Supercritical Fluid Chromatography on Polysaccharide-Based Stationary Phases
David Speybrouck1, Emmanuelle Lipka2
1Oril Industrie, Chemical and Analytical Development, Bolbec, France.
Abstract:
In the contemporary landscape, polysaccharide-based chiral stationary phases have garnered significant popularity for several reasons: (i) their broad application range, (ii) the availability of diverse chemistry options in both coated and immobilized forms, and (iii) their substantial loading capacity, advantageous for preparative-scale operations. Undoubtedly, chiral separations continue to be a focal point, especially within the pharmaceutical sector, where supercritical fluid chromatography is swiftly gaining traction. However, its utilization presents greater complexity compared to high-performance liquid chromatography. The provided examples serve to elucidate the development of analytical-scale chiral separation methods and the impact of each operational parameter, such as flow rate, outlet pressure, and temperature variations, on chiral separation.
More Related Videos
11:05Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
08:56Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Ion-Exchange Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...