Optimized Lentiviral Vector Production Using Dual-Pseudotyping with VSV-G and Sendai Virus HN Glycoproteins for...

Highly efficient gene delivery using lentiviral vectors is beneficial not only for basic cell science research but also for human gene therapy applications. Lentiviral pseudotyping with vesicular stomatitis virus G (VSV-G) is a commonly used method that enables the transduction of various cell types. However, the low expression of low-density lipoprotein-receptor, the primary receptor of VSV-G, limits the efficiency of lentiviral vector transduction. Sendai virus hemagglutinin-neuraminidase glycoproteins recognize terminal sialic acids on the host cell plasma membrane, facilitating viral entry. Here, we describe methods for lentiviral dual-pseudotyping with VSV-G and Sendai virus hemagglutinin-neuraminidase to broaden viral tropism.