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Lentivirus-Mediated Measurement of Transcription Factor Activity in the Brain

Kentaro Abe1,2

  • 1Lab of Brain Development, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan. k.abe@tohoku.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2025
PubMed

Keywords:
Lentiviral vectorQuantitative PCRRT-PCRTranscription factorTranscription reporter

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Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...