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Related Experiment Video

Updated: Jan 10, 2026

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Birth Date-Dependent Tracing of Neuronal Cell Lineage.

Tatsumi Hirata1

  • 1Brain Function Laboratory, National Institute of Genetics, SOKENDAI, Mishima, Shizuoka, Japan. tathirat@nig.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2025
PubMed
Summary

Neurogenic tagging combined with lentivirus injection precisely identifies specific neuron subsets. This method reveals convergent axon projections from olfactory bulb neurons in adult mice.

Keywords:
Birth timingLineageMouseNeurogenic taggingNeuron

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Area of Science:

  • Neuroscience
  • Developmental Biology

Background:

  • Neuronal phenotypes are influenced by cell lineage and developmental timing.
  • Classifying distinct neuron subsets is crucial for understanding neural circuits.
  • Existing methods require refinement for precise spatial and temporal control.

Purpose of the Study:

  • To describe a refined method for classifying and manipulating specific neuron subsets.
  • To spatially constrain neurogenically tagged neurons using focal lentivirus injection.
  • To identify convergent axon projections of specific olfactory bulb neuron subsets.

Main Methods:

  • Utilizing the "neurogenic tagging" method to control cell lineage and birth timing.
  • Combining neurogenic tagging with focal lentivirus injection for spatial constraint.
  • Preparing neurogenic-tagged mice for subsequent lentivirus delivery.

Main Results:

  • Successful spatial constraint of tagged neurons.
  • Identification of convergent axon projection patterns.
  • Demonstration of a specific subset of olfactory bulb neurons projecting convergently.

Conclusions:

  • The combined neurogenic tagging and focal lentivirus injection method enables precise targeting of neuron subsets.
  • This technique facilitates the study of specific neuronal populations and their projections.
  • The approach is valuable for investigating olfactory bulb circuitry and neuronal development.