Lentiviral Vector-Mediated Gene Delivery for iPSC-Related Neuroscience Research
Taro Okunomiya1,2, Keiko Imamura1,2,3, Taku Kashiyama4
1Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto, Japan.
Abstract:
The induced pluripotent stem cell (iPSC) technology provides human neural cells unprecedented material for neuroscience research. Lentiviral vectors have the advantage of being able to transfer genes into nondividing cells, which is useful for the identification and purification of specific neurons generated from human iPSCs, as well as for analysis of their function. Furthermore, the lentiviral vector has long-term expression and large gene capacity. This chapter describes protocols for lentiviral generation, labeling of iPSC-derived motor neurons with fluorescent proteins, and imaging of synaptic function.
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