Related Experiment Video
Updated: Jan 10, 2026
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Published on: December 10, 2014
Identification of molecular markers associated with comb traits through genome-wide association study on a
Yunjie Tu1, Shenghai Xu2, Yifan Liu1
1Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Yangzhou 225125, Jiangsu, China; Jiangsu Institute of Poultry Science, Yangzhou 225125, Jiangsu, China.
Abstract:
It is of great significance to identify candidate genes and molecular markers related to the comb development, in order to accelerate comb breeding progress of yellow feathered broiler. Herein, 400 terminal male chickens of the fast-type yellow feathered broiler (60 day old on the market) were selected as the research subjects, and their genomic genetic variation information was obtained using resequencing and genome-wide association study (GWAS). We identified 4 SNPs and 80 SNPs that were significantly correlated and potentially significantly correlated with comb height. Meanwhile, 4 SNPs and 5 SNPs were potentially significantly correlated with comb thickness and comb length, respectively. Several candidate genes related to comb traits were identified, including AR, HSF3, PIP4K2A, ATF6β, NTM, LRP1, etc. Except for SNP6 (rs317557994) locus with low genetic diversity, the other 12 SNP loci have moderate genetic diversity. Linkage disequilibrium (LD) analysis showed that 13 SNP loci were not in a strong linkage state. The association analysis between single marker and comb traits showed that AA and GA at SNP1 (rs312669246) locus, CC and CT at SNP2 (rs313331126) locus, AA and GA at SNP3 (rs313205409) locus, and AA and GA at SNP4 (rs314830898) locus were the dominant genotypes for comb height. The TT and CT genotypes at SNP5 (rs312557738), AA at SNP6 (rs317557994), GG at SNP7 (rs738748642), and AA and GA at SNP8 (rs312962270) were the dominant genotypes for comb thickness. TT at SNP9 (rs313797960), AA at SNP10 (rs312371971), AA at SNP11 (rs313637016), CC and CA at SNP12 (rs738049404), and GG at SNP13 (rs3388194039) were all the dominant genotypes for comb length. In chicken breeding, it is expected to assist in improving the selection of comb traits and accelerating the generation progress of comb development by selecting dominant genotypes at SNP1-SNP13 loci and eliminating other disadvantaged genotypes. These findings provide important references for further studying the regulatory mechanisms of comb development and cultivating new high-quality broiler breeds (crossed lines) with larger comb.
Related Concept Videos
00:49Complementation Tests
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
02:41Lethal Alleles
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
02:34DNA Microarrays
02:33Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
01:11Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...