Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Growth Curve01:28

Bacterial Growth Curve

2.1K
The bacterial growth curve is a fundamental concept in microbiology that describes the dynamics of bacterial population growth in a closed system with controlled environmental conditions, such as temperature and nutrient availability. This curve is divided into four distinct phases: lag, log (exponential), stationary, and death phases, each reflecting a unique stage of bacterial adaptation and growth. During the lag phase, bacteria acclimate to their surroundings by synthesizing essential...
2.1K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

536
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
536
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

1.5K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.5K
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

1.3K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Age-based approach to characterize the dynamics of cellular processes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Systematic discovery of enzyme promiscuity in Escherichia coli using in vitro metabolomics.

Communications biology·2026
Same author

Vsb1, Ypq1, and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole.

Life science alliance·2026
Same author

Metabolic thermodynamics: pertinent reference state and energy potentials.

The FEBS journal·2026
Same author

The return of metabolism: biochemistry and physiology of glycolysis.

Biological reviews of the Cambridge Philosophical Society·2025
Same author

Rubisco is slow across the tree of life.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jan 10, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K

Resolving misconceptions and constraints in growth-coupled bioproduction.

Lena M Hümmler1, Stefan Hristov1, Lucas Hille1

  • 1Department of Biochemistry, Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität, Berlin, Germany.

Current Opinion in Biotechnology
|November 22, 2025
PubMed
Summary
This summary is machine-generated.

Growth-coupled bioproduction (GCBP) ensures high yields and genetic stability by linking metabolic activity to product formation. This approach overcomes genetic drift and enhances bioproduction efficiency.

More Related Videos

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.0K
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.5K

Related Experiment Videos

Last Updated: Jan 10, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.6K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.0K
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.5K

Area of Science:

  • Metabolic Engineering
  • Synthetic Biology
  • Industrial Biotechnology

Background:

  • Growth-coupled bioproduction (GCBP) links cellular metabolism to product formation, aiming for high yields and stability.
  • Misconceptions exist regarding GCBP's stoichiometric constraints, cell division requirements, and compatibility with fermentation processes.
  • Genetic drift in microbial populations can reduce bioproduction efficiency.

Purpose of the Study:

  • Clarify persistent misconceptions about GCBP.
  • Highlight GCBP's advantages in ensuring high minimal yields and genetic stability.
  • Discuss challenges and future directions for GCBP implementation.

Main Methods:

  • Metabolic engineering principles applied to create growth-coupled systems.
  • Analysis of stoichiometric constraints and genetic stability mechanisms.
  • Evaluation of GCBP compatibility with various bioproduction strategies.

Main Results:

  • GCBP enforces an obligatory link between metabolic activity and product synthesis, ensuring high minimal yields.
  • Product yield is primarily limited by pathway constraints, not the growth-restoring enzyme.
  • GCBP is compatible with multistage fermentation and mitigates production losses from genetic drift.

Conclusions:

  • GCBP offers a robust strategy for continuous bioproduction by establishing high minimal stoichiometric yields.
  • Overcoming challenges in nonmodel organisms and computational design will further enhance GCBP.
  • Integrating GCBP with advanced metabolic engineering and computational tools can revolutionize industrial biotechnology.