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Published on: November 8, 2018
A high-resolution approach for cerebrospinal fluid cytokine detection using push-pull sampling and nano dot blot:
Salvador M Martinez-Gallegos1, Alberto Morales-Villagrán2, Laura Medina-Ceja1
1Laboratory of Neurophysiology, Department of Cellular and Molecular Biology, CUCBA, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Background:
TNF-α is a key proinflammatory cytokine implicated in the initiation and progression of neuroinflammatory responses. However, conventional detection techniques pose sample volume limitations that hinder the temporal resolution that can be achieved using animal models.
New Method:
In this study, we developed a novel system designed to quantify cytokines via continuous sampling of cerebrospinal fluid (CSF) via low-flow pushpull perfusion coupled with immunodetection via nanodot blotting. The system was tested in a 4-aminopyridine (4-AP) model of acute epileptiform activity, and the time course of TNF-α was characterized with a 1-min temporal resolution.
Results:
We observed rapid release of TNF-α within minutes of 4-AP administration. The peak of this release coincided temporally with the onset of epileptiform discharge trains, highlighting the relevance of TNF-α in the early phase of seizurelike activity. TNF-α levels returned to baseline within the first hour, a dynamic that would likely be missed by conventional low temporal resolution methodologies.
Comparison With Existing Methods And Conclusions:
Our findings demonstrate the utility of this minute-by-minute approach in capturing transient neuroinflammatory events and suggest that TNF-α may play a role in the initiation or modulation of acute epileptiform activity.
