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Updated: Aug 1, 2026
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Advanced surface-functionalized optical fiber biosensing platform for ultrasensitive fructose quantification in
Lan Yang1, Yansong Li2, Ruiduo Wang3
1Department of Anesthesiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi Province, China; Photonics Functional Materials and Devices Research Laboratory, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, Shaanxi, China.
A novel fiber sensor rapidly and accurately quantifies blood fructose levels, crucial for diagnosing diseases. This label-free biosensor shows high sensitivity and specificity, offering a low-cost clinical tool.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensing Technology
Background:
- Fructose concentration in human blood is clinically significant for disease diagnosis.
- Existing methods for rapid quantitative fructose analysis face limitations.
- Development of sensitive and specific detection methods is needed.
Purpose of the Study:
- To develop a novel fiber sensor for rapid and accurate quantitative analysis of fructose in human blood.
- To immobilize ketohexokinase (KHK) on a fiber sensor for specific fructose detection.
- To evaluate the sensor's performance in terms of sensitivity, specificity, and anti-interference capabilities.
Main Methods:
- Fabrication of a cascaded fiber sensor using microsphere cavities and flame tapering.
- Chemical immobilization of ketohexokinase (KHK) to create a specific biological functional layer.
- Spectral measurements of fructose standards and serum samples, validated against LC-MS.
Main Results:
- The optimized fiber sensor achieved a sensitivity of 5.98 nm/(μg/mL) and a detection limit of 12.6 ng/mL.
- The sensor demonstrated strong specificity and anti-interference capabilities in human serum samples.
- Bland-Altman analysis confirmed the sensor's accuracy and validity compared to LC-MS.
Conclusions:
- The developed fiber sensor provides a rapid, label-free, and cost-effective method for clinical fructose detection.
- Minimal sample volume and high accuracy make it suitable for widespread clinical application.
- This technology holds significant potential for improving disease diagnosis and management through precise fructose monitoring.
