Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

479
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
479
  1. Home
  3. Zebrafish Yolk High Phosphoprotein-derived Peptide Pt5-1c Inhibits Methicillin-resistant Staphylococcus Aureus By Targeting The Cell Membrane With Associated Ribosomal Perturbation.
  1. Home
  3. Zebrafish Yolk High Phosphoprotein-derived Peptide Pt5-1c Inhibits Methicillin-resistant Staphylococcus Aureus By Targeting The Cell Membrane With Associated Ribosomal Perturbation.

Related Experiment Video

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
10:45

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae

Published on: July 30, 2012

15.2K

Zebrafish yolk high phosphoprotein-derived peptide Pt5-1c inhibits methicillin-resistant Staphylococcus aureus by

Yazhuo Zhang1, Jia Liu1, Gehui Ren1

  • 1Lin He's Academician Workstation of New Medicine and Clinical Translation in Jining Medical University, Jining Medical University, Jining, 272067, China.

Fish & Shellfish Immunology
|November 23, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
Cell membraneMethicillin-resistant Staphylococcus aureusPhosvitin

More Related Videos

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection
10:04

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

Published on: January 7, 2019

7.4K
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
11:18

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Published on: March 15, 2012

44.5K

Related Experiment Videos

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
10:45

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae

Published on: July 30, 2012

15.2K
A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection
10:04

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

Published on: January 7, 2019

7.4K
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
11:18

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Published on: March 15, 2012

44.5K

Pt5-1c, an antibacterial peptide, effectively inhibits methicillin-resistant Staphylococcus aureus (MRSA) growth by disrupting its cell membrane. This peptide shows potential as a novel therapeutic agent against resistant bacterial infections.

Area of Science:

  • Biochemistry
  • Microbiology
  • Molecular Biology

Background:

  • Pt5-1c is an alpha-helical antibacterial peptide derived from zebrafish yolk high phosphoprotein.
  • Previous studies explored Pt5-1c's effects on common bacteria, but its impact on methicillin-resistant Staphylococcus aureus (MRSA) was unknown.

Purpose of the Study:

  • To investigate the specific effects of Pt5-1c on the growth and mechanisms of MRSA.

Main Methods:

  • MRSA was used as a model organism.
  • RNA-sequencing (RNA-seq) with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed.
  • Molecular docking was utilized to assess peptide-ribosome interactions.
  • Cell membrane permeability, depolarization, and intracellular content leakage were measured.

Main Results:

  • Pt5-1c significantly inhibited MRSA proliferation.
  • RNA-seq revealed upregulation of ribosome-related genes, but molecular docking showed weak, non-specific binding to the 70S ribosome.
  • Pt5-1c induced cell membrane permeability changes, depolarization, and leakage of intracellular contents, leading to cell lysis.

Conclusions:

  • Pt5-1c exhibits potent antibacterial activity against MRSA.
  • The primary mechanism involves direct damage to the bacterial cell membrane, leading to lysis, rather than ribosome inhibition.
  • Pt5-1c represents a promising candidate for developing new treatments against MRSA infections.