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Updated: Jan 10, 2026
Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
Fe/Mn bimetal-doped ZIF-8-encapsulated chitosanase for highly efficient synthesis of chitosan oligosaccharide
Xiangyu Zi1, Xiaolong Sun2, Fengwei Yin2
1Taizhou Key Laboratory of Biomass Functional Materials Development and Application, School of Life Science, Taizhou University, Taizhou, Zhejiang, 318000, China; School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, Jiangsu, China.
Abstract:
MOFs represent a promising platform for enzyme immobilization, yet the development of tailored strategies to accommodate both enzyme properties and MOF structures remains a challenge. In this study, we employed chitosanase BCC1 as a model enzyme and integrated Fe2+/Mn2+ ions into the ZIF-8 to construct a bimetallic MOF-based immobilization system (BCC1@Fe/Mn-ZIF-8). Comprehensive characterization by SEM, FTIR, TGA confirmed the successful immobilization and structural integrity of the composite. The immobilized enzyme retained 97 % of its initial activity and exhibited enhanced stability under harsh thermal, pH, and solvent conditions. Notably, BCC1@Fe/Mn-ZIF-8 maintained 117 % activity after 14 days at 4 °C and 72 % residual activity after 14 reuse cycles, indicating excellent storage and reusability. This bimetallic MOF-based strategy offers a robust and efficient approach for enzyme immobilization, with strong potential in industrial biocatalysis.
