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Updated: Jan 10, 2026
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Published on: June 6, 2025
Production of high-nutrient protein sources by pre-secondary solid-state fermentation of functional microbiota
Lian Zhang1, Xiaojuan Shen2, Zhi Wang1
1School of Chemical Engineering, Zhengzhou University, No. 100 Science Avenue, Zhengzhou 450001, China; State Key Laboratory of Biobased Transport Fuel Technology, Zhengzhou University, No. 100 Science Avenue, Zhengzhou 450001, China.
Abstract:
Chinese distillers grains (CDGs) are used as low-cost feed additives, because they have issues such as high crude fiber content (> 24 %), low crude protein content (< 17 %), and low digestibility (< 16 %). This study aimed to evaluate whether pre-secondary solid-state fermentation using functional microbiota can improve protein digestibility and reduce fiber content in CDGs. Results indicated that compared to CDGs, both true and crude protein content in solid-state fermented DCDGs (SSF-DCDGs) showed an upward trend, reaching increases of over 24 %. Compared to DCDGs, crude fiber content decreased; dry matter and protein digestibility increased by 29.54 % and 54.57 % in SSF-DCDGs. Amino acid content rose by 14.92 %, with glycine and alanine increased by 15.36 % and 21.25 % respectively-both being important aromatic flavor compounds. Therefore, the Pre-Sec-SSF-FM technical route can convert low-cost CDGs into a high-nutritional protein sources, offering a novel approach for protein raw material sourcing.
