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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
GmUbiE3 as a novel regulator of soybean seed size and weight identified via cross-population QTL analysis and
Shilin Luo1, Mingming Duan1, Bo Chen1
1Guangdong Subcenter of National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou 510642, China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou 510642, China.
Abstract:
Seed size and weight are critical yield-determining traits in soybean, yet their underlying genetic mechanisms remain incompletely clarified. To address this, we developed a newly expanded recombinant inbred lines (RILs) population, designated H3G, derived from selected lines of the GB13 RIL population established in our previous study. Employing multiple quantitative trait loci (QTL) mapping models and cross-population comparative analyses, we identified nine core genomic regions associated with seed size and weight traits. Finally, through systematic candidate gene identification and functional validation, we confirmed GmUbiE3 as a novel negative regulator of seed size and weight. CRISPR/Cas9-mediated knockout of GmUbiE3 resulted in a significant 28.07 % increase in soybean hundred-seed weight. These findings provide fundamental insights into the genetic control of soybean seed size and weight and offer valuable targets for molecular breeding approaches aimed at soybean yield improvement.
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