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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Cell Type-specific Gene Expression Profiling in the Mouse Liver
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In vivo cell-type specific secretome profiling.

Jie Liu1, Toren Finkel1, Shihui Liu2

  • 1Aging Institute of University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA, United States; Division of Cardiology, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

Methods in Cell Biology
|November 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method using proximity biotinylation to identify tissue-specific secreted proteins. This technique, applied to a new "Secretome Mouse" model, successfully profiled endothelial and muscle proteins, advancing proteomic research.

Keywords:
BioIDBiotinEndothelial cellMuscleProximity biotinylationSecretome

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Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Serum proteome complexity arises from diverse cellular and tissue origins.
  • Identifying tissue-specific contributions to the circulating proteome remains challenging.

Purpose of the Study:

  • To develop and validate a method for profiling tissue-specific secreted proteins.
  • To establish a mouse model for in vivo secretome elucidation.

Main Methods:

  • Utilized proximity biotinylation of endoplasmic reticulum-transiting proteins for in vitro secretome profiling.
  • Developed a "Secretome Mouse" model for in vivo tissue-specific secretome analysis.

Main Results:

  • Successfully identified in vivo endothelial-specific protein secretion.
  • Characterized muscle-derived serum proteins influenced by exercise using the model.
  • Demonstrated broad applicability of the Secretome Mouse model.

Conclusions:

  • Proximity biotinylation is effective for secretome profiling.
  • The Secretome Mouse model enables in vivo tissue-specific secretome analysis.
  • This approach advances the understanding of circulating proteins and their tissue origins.