Murine adult cardiomyocytes isolation and AAV transduction for secretome profiling using proximity labeling

In the heart, interaction among cells is essential for homeostasis and response to injury. Identifying paracrine/autocrine factors released by cardiomyocytes is fundamental to understand this complex network. However, limitations in the tools available to culture and manipulate isolated adult cardiomyocytes have hindered the study of these released factors. Here, we outline a protocol for profiling the secretome from isolated and in vitro cultured adult cardiomyocytes, using the well-established proximity ligation system.