Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CSF proteome-wide study of neuropsychiatric symptoms of dementia.

medRxiv : the preprint server for health sciences·2026
Same author

SECmeres outperform extracellular vesicles as potential blood RNA biomarkers for Alzheimer's disease.

Nature communications·2026
Same author

Plasma Proteomic Networks Reveal Shared Biology with Brain Linked to Alzheimer's Disease Pathology.

medRxiv : the preprint server for health sciences·2026
Same author

AMPK-mediated autophagy induction by bisdemethoxycurcumin attenuates senescence and amyloid pathology in 3xTg-AD mice.

Autophagy reports·2026
Same author

Integrating dorsolateral prefrontal cortex multi-omics and GWAS summary data reveals genetic etiology of Parkinson's disease.

medRxiv : the preprint server for health sciences·2026
Same author

Integrating dorsolateral prefrontal cortex multi-omics and GWAS summary data reveals genetic etiology of Parkinson's disease.

Research square·2026

Related Experiment Video

Updated: Jan 10, 2026

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
07:39

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

3.3K

Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models.

Prateek Kumar1, Dilpreet Kour1, Rashmi Kumari1

  • 1Department of Neurology, School of Medicine, Yale University, New Haven, CT, United States.

Methods in Cell Biology
|November 23, 2025
PubMed
Summary

This study introduces cell type-specific in vivo biotinylation of proteins (CIBOP) to analyze native proteomes in mouse brain tissue. CIBOP enables detailed proteomic analysis of specific cell types without isolation, advancing disease mechanism research.

Keywords:
BiotinylationGliaMouse modelsNeurological diseaseNeuronsProteomicsProximity labeling

More Related Videos

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.2K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

1.2K

Related Experiment Videos

Last Updated: Jan 10, 2026

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
07:39

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

3.3K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.2K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

1.2K

Area of Science:

  • Neuroscience
  • Proteomics
  • Molecular Biology

Background:

  • Transcriptomic studies may miss crucial disease insights not reflected at the RNA level.
  • Understanding cell type-specific protein expression in native tissue is vital for disease research.

Purpose of the Study:

  • To develop and present protocols for cell type-specific in vivo biotinylation of proteins (CIBOP) in the mouse brain.
  • To enable proteomic analysis of distinct cell types within their native tissue environment.

Main Methods:

  • Utilizing a proximity labeling approach with biotin ligase TurboID expressed in specific cell types.
  • Enriching biotinylated proteins from bulk tissue homogenates, followed by mass spectrometry-based quantitative proteomics.
  • Demonstrating CIBOP in neurons and astrocytes using both adenovirus and transgenic methods.

Main Results:

  • Successful implementation of CIBOP for cell type-specific proteome generation in mouse brain.
  • The method allows for broad proteomic biotinylation within targeted cell populations.
  • Generated cell type-specific proteomes revealing protein profiles in native states.

Conclusions:

  • CIBOP is a versatile pipeline for studying cell type-specific proteomes in vivo.
  • This technique can be applied to various cell types and disease models, including neurological and non-neurological conditions.
  • CIBOP offers a powerful complement to transcriptomic studies for understanding disease mechanisms.