Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epitope-Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi-Epitope Bispecific Antibody Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Cell-Mimetic Antifouling Poly(ethylene glycol) Interface Enables Selective Capture and Viable Release of Circulating Tumor Cells.

The journal of physical chemistry. B·2026
Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026
Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026
Same author

Nanobiosensors and Artificial Intelligence Strategies for Glycan Profiling in Cancer Progression: A Critical Review.

ACS sensors·2026

Related Experiment Video

Updated: Jan 10, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.5K

Programmable Dual-Targeting Nano Bioconjugates: a Universal Bioconjugation Strategy to Enhance Single Molecule

Fahimeh Farokhinejad1, Will Anderson1, Xueming Niu1

  • 1Centre of Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland, Brisbane, QLD, 4072, Australia.

Small Methods
|November 24, 2025
PubMed
Summary

A new bioconjugation method uses bispecific antibody fragments to functionalize nanoparticles, improving stability and enabling sensitive detection of disease biomarkers for precision medicine.

Keywords:
SERSbioconjugationbispecific antibody fragmentsnanoparticlessingle molecule detection

More Related Videos

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

261
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.2K

Related Experiment Videos

Last Updated: Jan 10, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.5K
Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

261
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

11.2K

Area of Science:

  • Bioconjugation Chemistry
  • Nanomedicine
  • Biomarker Detection

Background:

  • Existing bioconjugation methods for nanomaterials face challenges in site specificity, orientation, stability, and biofouling.
  • Functionalizing nanoparticles is critical for advanced biomedical applications, including diagnostics and targeted therapies.

Purpose of the Study:

  • To develop a universal and robust bioconjugation strategy for functionalizing nanoparticles with bispecific antibody fragments (BsAb-Fs).
  • To enable precise control over nanoparticle functionalization for enhanced target binding and stability in biomedical applications.
  • To demonstrate the utility of this strategy for sensitive biomarker detection using surface-enhanced Raman scattering (SERS).

Main Methods:

  • Site-specific conjugation of high-affinity BsAb-Fs to methoxy-polyethylene glycol (mPEG)-coated nanoparticles.
  • Utilizing BsAb-Fs to maintain programmable target-binding site orientation for optimal surface presentation.
  • Application of the bioconjugated nanoparticles as active single-particle SERS nanotags for cytokine detection.

Main Results:

  • The BsAb-Fs bioconjugation strategy provides controlled orientation and enhances target binding compared to traditional methods.
  • Bioconjugated SERS nanotags exhibit reduced batch-to-batch variability and improved colloidal stability, leading to enhanced immunoassay performance.
  • Simultaneous detection of three key inflammatory cytokines (TNF-α, IL-12, IL-1β) achieved with attomolar sensitivity.
  • Clinical evaluation in patients with major depressive disorder demonstrated the potential for diagnostic applications.

Conclusions:

  • The developed universal bioconjugation strategy offers a robust platform for nanoparticle functionalization using BsAb-Fs.
  • This approach significantly improves SERS-based immunoassay performance and enables highly sensitive detection of multiple biomarkers.
  • The method holds promise for advancing diagnostics and personalized medicine, particularly in inflammatory disease monitoring.