Related Experiment Video
Updated: Jan 10, 2026
11:34
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
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RBP2GO 2.0: Integrating disease associations and sequence features to explore RNA-binding protein functions
Simona Cantarella1, Jule Neffe1, Malte Hermes1
1Research group "RNA-Protein Complexes & Cell Proliferation", German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.
Nucleic Acids Research
|November 24, 2025
Summary
RBP2GO 2.0 is an updated database of RNA-binding proteins (RBPs), their functions, and disease associations across 13 species. This resource aids researchers in exploring RBP roles in biology and human diseases.
Area of Science:
- Molecular Biology
- Bioinformatics
- Genomics
Background:
- RNA-binding proteins (RBPs) are crucial regulators of gene expression, impacting numerous biological processes and human diseases.
- Understanding RBP functions and interactions is essential for deciphering cellular mechanisms and disease pathogenesis.
Purpose of the Study:
- To update and enhance the RBP2GO database, providing a comprehensive resource for RNA-binding proteins (RBPs).
- To integrate new data, including disease ontology and sequence features, to improve the characterization of RBPs.
- To facilitate research into RBP functions and their links to human diseases.
Main Methods:
- Integration of updated proteome-wide RBP studies and new datasets (e.g., eCLIP/iCLIP from ENCODE, R-DeeP).
- Development of RBP2GO and RBP2GO Composite Scores to predict RNA-binding probability.
- Implementation of a redesigned user interface with enhanced search functionalities for proteins, gene ontology, and disease ontology terms.
Main Results:
- RBP2GO 2.0 now includes expanded information on disease ontology, linking RBP functions to human diseases.
- The database features novel visualizations of sequence features and RNA-binding peptides.
- New scoring systems (RBP2GO Score, RBP2GO Composite Score) and violin plot distributions enhance protein characterization.
Conclusions:
- RBP2GO 2.0 is a significantly improved and valuable resource for the scientific community.
- The database supports the investigation of novel RBP functions and their roles in health and disease.
- RBP2GO 2.0 is publicly accessible, promoting broader research and discovery in the field of RNA-binding proteins.
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