Tea Plant miR5368-p5 Negatively Regulates Resistance Against Lasiodiplodia theobromae Through Targeting the
Bin Wang1, Ruixin Zhang1, Dongqin Zhang1
1State Key Laboratory of Green Pesticide, Guizhou University, Guiyang, China.
Molecular Plant Pathology
|November 24, 2025
Summary
Tea plants
Area of Science:
- Plant Pathology
- Molecular Biology
- Genetics
Background:
- Tea plants are susceptible to Lasiodiplodia theobromae, causing significant crop losses.
- Understanding tea plant resistance mechanisms is crucial for disease management.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying tea plant resistance to Lasiodiplodia theobromae.
- To identify key genes and regulatory pathways involved in host defense.
Main Methods:
- Gene expression analysis (RT-qPCR)
- Gene silencing (AsODN)
- Transgenic plant assays (Nicotiana benthamiana)
- Yeast two-hybrid assays
- Bimolecular fluorescence complementation (BiFC)
- DNA affinity purification sequencing
- Electrophoretic mobility shift assay (EMSA)
- Dual-luciferase reporter assays
- Degradome sequencing
Main Results:
- CsWRKY57 enhances tea plant resistance to L. theobromae.
- CsWRKY57 interacts with CsTIFY5A, which negatively regulates resistance.
- CsWRKY57 activates CsLRR-RLK expression, promoting resistance.
- miR5368-p5 targets CsWRKY57, negatively impacting resistance.
Conclusions:
- The miR5368-p5-CsWRKY57-CsLRR-RLK module, involving CsTIFY5A, is critical for tea plant defense against L. theobromae.
- This study reveals novel insights into tea plant disease resistance pathways.
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