Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pre-post changes in agility, balance, and functional movement during short-term functional strength and balance training in national-level Kho-Kho players.

Scientific reports·2026
Same author

A retrospective observational study of placenta accreta spectrum perioperative anaesthesia management in a tertiary care centre: A 25-year experience.

Indian journal of anaesthesia·2026
Same author

Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin.

International journal of molecular sciences·2026
Same author

Replaced right hepatic artery arising directly from the celiac trunk in a difficult pancreaticoduodenectomy for intraductal papillary mucinous neoplasm after cholangitis and biliary stenting: a case report and literature review.

Journal of surgical case reports·2026
Same author

Knowledge, Attitudes, and Practices of Adult Vaccination Among Medical Students in Western India.

Cureus·2026
Same author

LncRNA YIYA drives pancreatic cancer proliferation under high-glucose conditions by reinforcing a RAS-PKM2-dependent Warburg phenotype.

FEBS letters·2026

Related Experiment Video

Updated: Jan 10, 2026

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
10:26

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Published on: December 19, 2014

38.4K

Enzyme-assisted extraction of leaf proteins: efficiency, functionality, and structural insights.

Ankita Sharma1, Shalini Sharma2, Godasritha Ramaraju1

  • 1Department of Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab 144411, India.

Food Chemistry: X
|November 24, 2025
PubMed
Summary

Enzyme-assisted extraction (EAE) enhances green leaf protein recovery. This method improves protein functionality for food applications compared to traditional alkaline extraction.

Keywords:
Enzyme-assisted extractionFunctional propertiesLeaf proteinProcess optimisationProtein characterisationProtein extraction

More Related Videos

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.5K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

18.1K

Related Experiment Videos

Last Updated: Jan 10, 2026

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
10:26

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Published on: December 19, 2014

38.4K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.5K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

18.1K

Area of Science:

  • Food Science
  • Biotechnology
  • Plant Biochemistry

Background:

  • Leaf proteins offer a sustainable and nutritionally valuable alternative protein source.
  • Conventional extraction methods often have limitations in efficiency and protein quality.
  • Enzyme-assisted extraction (EAE) utilizes enzymatic hydrolysis to improve compound release from plant matrices.

Purpose of the Study:

  • To review the application of EAE for green leaf protein extraction.
  • To identify optimal process parameters for EAE.
  • To compare the characteristics and functional properties of EAE-derived leaf proteins with those from alkaline extraction.

Main Methods:

  • Literature review focusing on enzyme-assisted extraction techniques for leaf proteins.
  • Analysis of enzyme types (proteases, xylanases, etc.) and their roles in cell wall degradation.
  • Comparative evaluation of protein properties based on extraction methods.

Main Results:

  • EAE effectively degrades plant cell walls, enhancing leaf protein release.
  • Enzyme-assisted extracted leaf proteins exhibit improved functional properties.
  • EAE offers a greener alternative to conventional alkaline extraction methods.

Conclusions:

  • Enzyme-assisted extraction is a promising technology for high-quality green leaf protein production.
  • Optimized EAE parameters can yield leaf proteins with enhanced functional attributes.
  • These proteins show potential as functional food ingredients, including additives and nutritional enhancers.