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CYClones: A highly powered, fully genotyped, 8-parent yeast mapping population.
Gareth A Cromie1, Russell S Lo1, Trey S Morgan1
1Pacific Northwest Research Institute, Seattle, Washington, USA.
Biorxiv : the Preprint Server for Biology
|November 24, 2025
Summary
A new yeast resource, CYClones (Collaborative Yeast Cross clones), was created from 11,392 segregants. This powerful tool aids in dissecting complex traits and understanding genetic diversity in Saccharomyces cerevisiae.
Area of Science:
- Microbiology
- Genetics
- Yeast Research
Background:
- The budding yeast *Saccharomyces cerevisiae* exhibits significant genetic diversity across natural isolates.
- Understanding this diversity is key to dissecting complex traits and evolutionary adaptations.
Purpose of the Study:
- To construct and characterize CYClones, a large library of yeast segregants for genetic studies.
- To enable high-resolution mapping of quantitative trait loci (QTLs) and identify causal variants.
Main Methods:
- Generation of 11,392 segregants from a multiparental cross of eight diverse *S. cerevisiae* strains.
- Imputation of whole-genome sequences for all segregants.
- Assessment of haplotype representation and genetic diversity captured by the library.
Main Results:
- CYClones captures a substantial fraction of global *S. cerevisiae* genetic diversity.
- Haplotype representation is well-maintained across the genome.
- Simulations show high power (≥95%) for detecting variants with low heritability and fine mapping resolution.
Conclusions:
- CYClones is a valuable community resource for yeast genetic research.
- Facilitates the dissection of complex traits and identification of causal variants.
- Enables the study of context-dependent mutational effects in *S. cerevisiae*.
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