Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Karyotyping

Karyotyping

Karyotyping

Karyotyping

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genetic analysis of imaging-derived phenotypes.

Nature reviews. Genetics·2026

Same author

CYClones: A highly powered, fully genotyped, 8-parent yeast mapping population.

G3 (Bethesda, Md.)·2026

Same author

Functional profiling of 2,193 ASS1 missense variants: Insights into variant pathogenicity and epistatic interactions in citrullinemia type I.

PLoS genetics·2026

Same author

An interpretable machine learning framework for dog breed inference and ancestry decomposition.

bioRxiv : the preprint server for biology·2026

Same author

Blood biomarkers and breed genetics of aging in pet dogs.

bioRxiv : the preprint server for biology·2026

Same author

Disordered but Different: Functional and Evolutionary Divergence of Transcription Factor Intrinsically Disordered Regions.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: May 12, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

CYClones: A highly powered, fully genotyped, 8-parent yeast mapping population.

Gareth A Cromie¹, Russell S Lo¹, Trey S Morgan¹

¹Pacific Northwest Research Institute, Seattle, Washington, USA.

Biorxiv : the Preprint Server for Biology

|November 24, 2025

Summary

A new yeast resource, CYClones (Collaborative Yeast Cross clones), was created from 11,392 segregants. This powerful tool aids in dissecting complex traits and understanding genetic diversity in Saccharomyces cerevisiae.

Keywords:

Budding yeast Funnel Cross Mapping population Multi-parent advanced generation intercross

More Related Videos

BEST: Barcode Enabled Sequencing of Tetrads

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

Related Experiment Videos

Last Updated: May 12, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

BEST: Barcode Enabled Sequencing of Tetrads

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

Area of Science:

Microbiology
Genetics
Yeast Research

Background:

The budding yeast *Saccharomyces cerevisiae* exhibits significant genetic diversity across natural isolates.
Understanding this diversity is key to dissecting complex traits and evolutionary adaptations.

Purpose of the Study:

To construct and characterize CYClones, a large library of yeast segregants for genetic studies.
To enable high-resolution mapping of quantitative trait loci (QTLs) and identify causal variants.

Main Methods:

Generation of 11,392 segregants from a multiparental cross of eight diverse *S. cerevisiae* strains.
Imputation of whole-genome sequences for all segregants.
Assessment of haplotype representation and genetic diversity captured by the library.

Main Results:

CYClones captures a substantial fraction of global *S. cerevisiae* genetic diversity.
Haplotype representation is well-maintained across the genome.
Simulations show high power (≥95%) for detecting variants with low heritability and fine mapping resolution.

Conclusions:

CYClones is a valuable community resource for yeast genetic research.
Facilitates the dissection of complex traits and identification of causal variants.
Enables the study of context-dependent mutational effects in *S. cerevisiae*.