Characterization of Gait Kinematics and Muscle Function in Becker Muscular Dystrophy Pigs: a pilot study

Area of Science:

• Biomedical Science
• Animal Models
• Musculoskeletal Research

Background:

• Becker muscular dystrophy (BMD) is a genetic disorder causing progressive muscle weakness.
• Developing accurate animal models is crucial for understanding BMD and testing treatments.
• Previous vertebrate models have limitations in mimicking the human condition.

Purpose of the Study:

• To characterize the gait kinematics and muscle function of a naturally occurring pig model of BMD.
• To evaluate the potential of this pig model for preclinical therapeutic assessment.

Main Methods:

• Gait kinematics and range of motion (ROM) were analyzed in BMD pigs.
• Muscle function, including isometric force and fatigue index, was measured in flexor and extensor muscles.
• Histological analysis assessed muscle fiber cross-sectional area and dystrophin protein levels.

Main Results:

• BMD pigs showed altered hip ROM and reduced isometric force in flexor muscles.
• Flexor muscles exhibited a significant increase in the fatigue index and decreased dystrophin levels.
• Histology revealed changes in muscle fiber size distribution, consistent with a BMD phenotype in flexor muscles.

Conclusions:

• The naturally occurring BMD pig model displays key characteristics of the human condition.
• This model demonstrates reduced muscle function and specific histological changes relevant to BMD.
• The characterized BMD pig model is a potentially valuable tool for preclinical therapeutic efficacy and safety studies.