Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet...

Area of Science:

• Microscopy
• Biophysics
• Optical Engineering

Background:

• Light-Sheet Fluorescence Microscopy (LSFM) offers gentle, rapid, and efficient volumetric imaging.
• LSFM variants are often narrowly optimized, limiting broad applicability.
• A need exists for adaptable LSFM platforms for diverse biological samples.

Purpose of the Study:

• Introduce the multi-immersion oblique plane microscope (miOPM) as an adaptable LSFM platform.
• Demonstrate miOPM's capability for high-resolution imaging across various spatial scales.
• Expand the adaptability and ease of use of LSFM for advanced 3D imaging.

Main Methods:

• Developed the multi-immersion oblique plane microscope (miOPM).
• Enabled seamless interchangeability of oil, water, and air objectives.
• Validated performance across a refractive index range of 1.33 - 1.51.

Main Results:

• miOPM supports high-resolution imaging from subcellular dynamics to whole organisms and cleared tissues.
• Achieved diffraction-limited performance across a wide refractive index range.
• Demonstrated compatibility with standard sample mounting, clearing protocols, and high-throughput 3D imaging.

Conclusions:

• The miOPM significantly expands the adaptability and ease of use of LSFM.
• miOPM facilitates high-resolution 3D imaging of diverse biological specimens at multiple scales.
• This adaptable LSFM platform is poised to democratize advanced three-dimensional imaging.