Jove
Visualize
Contact Us

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.5K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.5K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

41.4K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.4K
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

335
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
335
Applications of RC Circuits01:22

Applications of RC Circuits

4.0K
A relaxation oscillator is one of the applications of RC circuits. A neon lamp relaxation oscillator comprises a capacitor, a resistor, a voltage source, and a lamp. The lamp acts like an open circuit, with infinite resistance until the potential difference across the lamp reaches a specific voltage. At that voltage, the lamp acts like a short circuit with zero resistance, and the capacitor discharges through the lamp, thus producing light. Once the capacitor is fully discharged through the...
4.0K
Sleep-Wake Cycles01:24

Sleep-Wake Cycles

2.7K
Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and  rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure to perfluorooctanoic acid accelerates <i>Drosophila melanogaster</i> juvenile development and disrupts mitochondrial metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Direct quantification of the metabolic heat output of individual Drosophila brains.

Cell reports methods·2026
Same author

SWI/SNF nuclear foci scaffold peripheral gene hubs for circadian chromatin control.

bioRxiv : the preprint server for biology·2026
Same author

The role of geography and regional climate in shaping the chemical profiles and biological potentials of Citrus essential oils.

Food chemistry·2025
Same author

Sequestration of clock proteins into repressive nuclear condensates orchestrates circadian gene repression.

bioRxiv : the preprint server for biology·2025
Same author

Direct quantification of the metabolic heat output of individual <i>Drosophila</i> brains.

bioRxiv : the preprint server for biology·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 10, 2026

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.3K

An intron-based timer for circadian rhythms.

Ye Yuan1, Amanda Linskens1, Rafael De Gouvea2

  • 1Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, 48109, USA.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

A novel RNA timer in the Drosophila timeless (tim) gene, intron P, controls circadian rhythms by regulating mRNA nuclear retention. This intron

More Related Videos

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.3K
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.7K

Related Experiment Videos

Last Updated: Jan 10, 2026

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

10.3K
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.3K
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
06:53

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

8.7K

Area of Science:

  • Chronobiology
  • Molecular Biology
  • RNA Biology

Background:

  • Circadian clocks govern ~24-hour rhythms in eukaryotes via transcriptional-translational feedback loops.
  • The precise molecular mechanisms setting the circadian period remain incompletely understood.

Purpose of the Study:

  • To identify novel molecular timers regulating circadian period length.
  • To investigate the role of RNA processing in circadian gene expression.

Main Methods:

  • Single-molecule imaging
  • Nascent RNA sequencing
  • CRISPR-mediated gene editing
  • Heterologous reporter assays in Drosophila and human cells

Main Results:

  • A single intron (intron P) in the Drosophila timeless (tim) gene acts as an RNA-based molecular timer.
  • Intron P causes nuclear retention of ~50% of tim mRNAs due to inefficient splicing, controlling period length.
  • Removal of intron P shortens the circadian period to ~22 hours; its insertion into other genes confers nuclear retention.
  • RNA-binding proteins (Hrb27C, Squid, Qkr58E-2) modulate intron P splicing kinetics.

Conclusions:

  • tim intron P is the first identified intron-based molecular timer in circadian clocks.
  • Splicing kinetics represent a critical regulatory layer in temporal gene expression.
  • This mechanism has potential implications for development and immunity.