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Updated: Jan 10, 2026

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
Published on: August 18, 2020
NPS neurons receive extensive input from auditory brainstem nuclei
Richie Zhang1, Joel C Geerling1
1Department of Neurology, University of Iowa.
None:
Neurons that produce NPS send output to brain regions implicated in circadian function and threat responses, but less is known about the afferent control of NPS neurons. In this study, we used a conventional retrograde tracer, cholera toxin beta subunit (CTb), to identify afferents to the rostral-lateral parabrachial region that contains the main concentration of NPS neurons. We then used Cre-dependent rabies retrograde tracing in Nps-2A-Cre mice to identify inputs specifically to NPS neurons. Nps-expressing neurons receive heavy input from auditory brainstem structures, including the inferior colliculus, nucleus of the lateral lemniscus, superior olivary complex, and cochlear nucleus. These findings suggest an unexpected role for auditory information in controlling the activity of NPS neurons.
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