Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

272
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
272

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative genomic analysis of <i>Bacteroides fragilis</i> from intestinal and extra-intestinal sites.

Microbiology spectrum·2026
Same author

Biofilm-derived curli and Z-DNA shape anti-DNA antibody responses during Salmonella infections.

PLoS pathogens·2026
Same author

Off-Label 16-mm VSD Occluder for Closure of a Giant PDA With Severe Pulmonary Hypertension.

JACC. Case reports·2026
Same author

Microglial-Derived IGF-1 Serves as a Regulator for Neuroimmune Homeostasis During Viral-Induced Demyelination.

Viruses·2026
Same author

Alkamines reveal a hidden layer of steroid and drug metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Chronic exposure to Salton Sea aerosols elicits pulmonary inflammation and shifts in murine lung and fecal microbiome diversity.

Research square·2026

Related Experiment Video

Updated: Jan 10, 2026

Application of a Mouse Ligated Peyer&#8217;s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
05:59

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

Published on: December 17, 2011

20.1K

Commensal Escherichia coli colonization triggers Peyer's patch development.

Romana R Gerner1,2,3, Gregory T Walker1, Suzi M Klaus4

  • 1Division of Host-Microbe Systems and Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary

Escherichia coli, a gut bacterium, drives B cell accumulation in the gut through curli fibers signaling via TLR2. This process, dependent on CXCR2, influences mucosal immune development beyond the neonatal period.

More Related Videos

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
08:53

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Published on: December 4, 2020

6.7K
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
09:49

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Published on: March 17, 2013

33.6K

Related Experiment Videos

Last Updated: Jan 10, 2026

Application of a Mouse Ligated Peyer&#8217;s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
05:59

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

Published on: December 17, 2011

20.1K
Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
08:53

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Published on: December 4, 2020

6.7K
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
09:49

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Published on: March 17, 2013

33.6K

Area of Science:

  • Immunology
  • Microbiology
  • Gastroenterology

Background:

  • The gut microbiota is crucial for mucosal immunity.
  • Specific microbes driving lymphoid tissue development are not well understood.

Purpose of the Study:

  • Identify microbes that promote B cell accumulation in gut-associated lymphoid tissues.
  • Elucidate the mechanisms by which these microbes influence immune development.

Main Methods:

  • Investigated the role of Escherichia coli in B cell recruitment.
  • Utilized a CXCR2-dependent mechanism and Toll-like receptor 2 (TLR2) signaling pathway.
  • Analyzed the impact of curli amyloid fibers produced by E. coli.

Main Results:

  • Escherichia coli was identified as a key driver of naïve B cell accumulation in Peyer's patches and lamina propria.
  • E. coli promotes B cell recruitment via curli amyloid fibers signaling through TLR2 and a CXCR2-dependent mechanism.
  • This microbial influence on immune development occurs over a broader temporal window than previously recognized.

Conclusions:

  • Escherichia coli plays a significant role in the development of gut-associated lymphoid tissue.
  • Curli amyloid fibers produced by E. coli are key molecular products mediating B cell recruitment.
  • Microbial modulation of mucosal immunity is a dynamic process with implications for immune development throughout life.