Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.2K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.2K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.3K
2.3K
The DNA Replication Fork01:02

The DNA Replication Fork

40.3K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.3K
The DNA Replication Fork01:02

The DNA Replication Fork

18.0K
18.0K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

60.8K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
60.8K
DNA Replication02:40

DNA Replication

58.2K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe.

G3 (Bethesda, Md.)·2026
Same author

A cardiovascular, craniofacial, and neurodevelopmental disorder caused by loss-of-function variants in the eIF3 complex component genes EIF3A and EIF3B.

American journal of human genetics·2026
Same author

A cardiovascular, craniofacial, and neurodevelopmental disorder caused by loss-of-function variants in the eIF3 complex component genes EIF3A and EIF3B.

American journal of human genetics·2025
Same author

Checkpoint-Dependent Sensitivities to Nucleoside Analogues Uncover Specific Patterns of Genomic Instability.

Current issues in molecular biology·2025
Same author

Diploidy confers genomic instability in Schizosaccharomyces pombe.

Genetics·2025
Same author

Diploidy confers genomic instability in <i>Schizosaccharomyces pombe</i>.

bioRxiv : the preprint server for biology·2025
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.6K

Modelling DNA replication fork stability and collapse using chromatin fiber analysis and the R-ODD-BLOBS program.

Kerenza Cheng1, Kazeera Aliar2, Roozbeh Manshaei3

  • 1Molecular Science Graduate Program, Yeates School of Graduate and Postdoctoral Studies, Toronto Metropolitan University, Toronto ON M5B 2K3.

Biorxiv : the Preprint Server for Biology
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

Replication fork anatomy was studied in Schizosaccharomyces pombe using R-ODD-BLOBS software. Checkpoint loss in mrc1Δ mutants increased Rad51 protein presence, suggesting more homologous recombination repair at these forks.

Keywords:
DNA recombinationDNA replication forkSchizoasaccharomyces pombehydroxyurea (HU) arrest

More Related Videos

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles
13:09

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Published on: March 3, 2023

4.8K
Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.2K

Related Experiment Videos

Last Updated: Jan 10, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

40.6K
Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles
13:09

Demonstration of the DNA Fiber Assay for Investigating DNA Damage and Repair Dynamics Induced by Nanoparticles

Published on: March 3, 2023

4.8K
Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication is a fundamental process, but its regulation and the role of associated proteins, especially during checkpoint activation or loss, are complex.
  • Understanding replication fork dynamics is crucial for comprehending genome stability and disease.
  • Schizosaccharomyces pombe is a model organism for studying cell cycle control and DNA replication.

Purpose of the Study:

  • To characterize the anatomy of DNA replication forks in wild-type and mutant Schizosaccharomyces pombe strains.
  • To investigate the distribution of Rad51 and Cdc45 proteins at replication forks and their correlation with DNA synthesis.
  • To assess the impact of checkpoint loss (mrc1Δ and cds1Δ mutations) on replication fork structure and protein association.

Main Methods:

  • Utilized BrdU labeling to measure synthesized DNA lengths at replication forks.
  • Employed chromatin fiber imaging and pixel intensity analysis.
  • Developed and applied the R-ODD-BLOBS computational tool for analyzing large datasets of chromatin spread data.
  • Quantified Rad51 and Cdc45 protein colocalization with replicated and unreplicated DNA regions.

Main Results:

  • Average BrdU tract lengths were measured: cds1Δ (~2.9 kb) > wild type (~2.5 kb) > mrc1Δ (~1.7 kb).
  • Rad51 protein was found in 22% more replicated areas in mrc1Δ mutants compared to wild type.
  • Cdc45 protein distribution was also analyzed in relation to replication fork status.

Conclusions:

  • The R-ODD-BLOBS tool is effective for analyzing chromatin spread data to understand DNA replication.
  • Checkpoint loss, particularly in mrc1Δ, alters replication fork structure and protein composition.
  • Increased Rad51 presence in mrc1Δ mutants suggests a higher reliance on homologous recombination repair pathways.