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Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Updated: Jan 10, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging.

Kevin T Uning^1,2, Henry Brisebois³, Conor C Horgan⁴

¹London Centre for Nanotechnology, University College London, London, UK.

Biorxiv : the Preprint Server for Biology

|November 24, 2025

Summary

This summary is machine-generated.

We present an Open Raman Microscopy (ORM) framework, an accessible, modular system for chemical fingerprinting. This open-source hardware and software solution enhances the reach of Raman microscopy in diverse scientific fields.

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Area of Science:

Chemical Imaging
Spectroscopy
Biomedical Research

Background:

Raman microscopy offers label-free, non-destructive chemical fingerprinting.
High cost and technical complexity limit accessibility of commercial and custom Raman systems.
Growing applications in materials science, environmental analysis, and biomedical research.

Purpose of the Study:

Introduce an Open Raman Microscopy (ORM) framework to improve accessibility.
Provide a modular, open-source hardware and software solution for Raman imaging.
Demonstrate the ORM platform's versatility across different scientific applications.

Main Methods:

Developed a modular microscopy platform for Raman imaging.
Created the ORM-Integrated Raman and Imaging Software (ORM-IRIS) for component control.
Implemented the ORM platform in three institutions for diverse imaging tasks.

Main Results:

Demonstrated high-throughput imaging of articular cartilage.
Showcased confocal 3D imaging of zebrafish embryos.
Successfully imaged gold colloid surfaces for surface-enhanced Raman spectroscopy.

Conclusions:

The ORM framework significantly enhances Raman microscopy accessibility.
Open-source hardware and software facilitate broader scientific adoption.
The platform supports diverse applications from tissue analysis to materials science.