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Updated: Jan 10, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
Published on: May 12, 2023
From loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2'-deoxyuridine
Jack Barr1, Tayler D Prieto Otoya1,2, Christine Cardin2
1Organic and Biomimetic Chemistry Research Group, Ghent University, Krijgslaan 281 S4 B-9000 Ghent Belgium Enrico.Cadoni@UGent.be.
Abstract:
We report the use of 5-furyl-2'-deoxyuridine (5FU) as a fluorescent probe to distinguish ligand binding to distinct G-quadruplex tetrads. Site-specific incorporation of 5FU into T95-2T at the capping regions enabled discrimination of peptide-binding events via fluorescence turn-on, providing insights into tetrad preference and binding affinities using the 5FU-G4/RHAU peptide system.
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