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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Light Acquisition02:16

Light Acquisition

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Fruit Development, Structure, and Function01:58

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Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Methods of Classification and Identification01:28

Methods of Classification and Identification

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Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
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Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

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Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
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Related Experiment Video

Updated: Jan 10, 2026

Fruit Volatile Analysis Using an Electronic Nose
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Machine learning classification of mango maturity based on carotene content from Raman spectra.

Ji Loun Tan1, Fazida Hanim Hashim1,2, Jahariah Sampe3

  • 1Department of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.

Peerj
|November 24, 2025
PubMed
Summary

Raman spectroscopy accurately determines mango ripeness by analyzing carotenoid compounds in the peel. This non-invasive method offers a reliable alternative to traditional assessments, ensuring optimal fruit quality and yield.

Keywords:
CaroteneMachine learningMangoRaman spectroscopyRipeness

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Area of Science:

  • Agricultural Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Mango ripeness is crucial for taste, aroma, and nutrition, impacting farmer yields.
  • Traditional ripeness assessments are inconsistent, inaccurate, and time-consuming.
  • Variations in mango color and human perception affect traditional methods.

Purpose of the Study:

  • Develop a non-invasive, efficient method for detecting mango ripeness using Raman spectroscopy.
  • Extract organic compound data from raw Raman spectra.
  • Correlate carotene characteristics with mango ripeness levels.
  • Evaluate machine learning models for mango ripeness classification.

Main Methods:

  • Analyzed 29 mango fruit spectra, with 13 samples representing underripe, ripe, and overripe categories.
  • Utilized Raman spectroscopy to analyze organic compounds in mango peel.
  • Applied statistical analysis and machine learning models (SVM, KNN) for classification.

Main Results:

  • Identified carotenoids (lycopene, β-carotene, lutein, neoxanthin) in mango peel (1,480–1,550 cm⁻¹).
  • Confirmed significant correlation between carotenoid peak intensity and mango maturity (p < 0.05).
  • Achieved 100% accuracy in classifying ripeness using SVM and KNN models.

Conclusions:

  • Raman spectroscopy is a reliable, robust, and non-invasive method for assessing mango ripeness.
  • The technique is immune to external factors like light, humidity, and noise.
  • This approach holds promise for improving mango quality control and harvest management.