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Related Experiment Videos

Chromosome replication in Myxococcus xanthus.

D R Zusman, D M Krotoski, M Cumsky

    Journal of Bacteriology
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    DNA synthesis in Myxococcus xanthus occurs throughout most of the cell cycle, regardless of generation time. Cells begin with one chromosome that replicates and segregates before cell division.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Cell Biology

    Background:

    • Understanding bacterial DNA replication and cell division is crucial for microbial genetics.
    • Myxococcus xanthus exhibits complex developmental behaviors, making its cell cycle regulation of interest.

    Purpose of the Study:

    • To investigate the kinetics of DNA synthesis during the cell division cycle of Myxococcus xanthus.
    • To determine the DNA content and chromosome number in Myxococcus xanthus at different stages of its life cycle.

    Main Methods:

    • Measurement of DNA synthesis rates across the cell cycle in varying growth media.
    • Determination of DNA content per cell using spectrophotometry.
    • Analysis of chromosome segregation using prelabeling and outgrowth experiments.

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  • Inhibition of protein synthesis with chloramphenicol to assess DNA content changes.

    • Main Results:

    • DNA synthesis occupied approximately 85% of the cell cycle, independent of generation times ranging from 5 to 11 hours.
    • Cells contain one nucleoid at birth, which divides synchronously mid-cycle, with segregation initiating before replication completion.
    • Exponential-phase cells had ~20 x 10(-9) microgram DNA, while newborn cells had ~14 x 10(-9) microgram.
    • Chloramphenicol treatment increased DNA content by ~50% in exponential-phase cells.
    • Cells are born with one unreplicated chromosome, estimated molecular weight ~8.4 x 10(9).

    Conclusions:

    • Myxococcus xanthus exhibits a simple DNA replication cycle tightly coupled to cell division.
    • The cell cycle is characterized by a single chromosome that replicates and segregates synchronously.
    • The data supports a model of cell division initiated with a single, complete, unreplicated chromosome.