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Updated: May 7, 2026

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
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Molecular detection and characterization of methicillin-resistant Staphylococcus pseudintermedius (MRSP) ST2361 in a

Reza Besharati1, Ali Haghbin2, Seyed Ahmad Hashemi3

  • 1Department of Pathobiology and Laboratory Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.

One Health (Amsterdam, Netherlands)
|November 24, 2025
PubMed
Abstract

Insights

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) was found in hospital staff and supplies in Iran. This highlights the risk of MRSP spreading between animals, humans, and healthcare settings.

Area of Science:

  • Veterinary Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Staphylococcus pseudintermedius is a common dog bacterium.
  • Methicillin-resistant strains (MRSP) are an emerging zoonotic threat.
  • This study focuses on MRSP in a hospital setting in Iran.

Purpose of the Study:

  • Investigate MRSP in hospital environments and healthcare workers.
  • Assess antimicrobial resistance and zoonotic potential of MRSP isolates.
  • Determine the prevalence and characteristics of MRSP in Bojnurd, Iran.

Main Methods:

  • Cultured nasal swabs from healthcare workers and hospital supplies.
  • Performed antimicrobial susceptibility testing (Kirby-Bauer).
  • Conducted PCR for resistance (mecA, vanA, cfr) and virulence genes (pvl, hla, icaA/D), SCCmec typing, and MLST.

Main Results:

  • Identified two MRSP isolates (ST2361), one from a healthcare worker and one from hospital supplies.
  • Isolates showed resistance to beta-lactams, fluoroquinolones, macrolides, and sulfonamide-trimethoprim.
  • Confirmed presence of mecA and virulence genes (pvl, hla, icaA/D); SCCmec type IV and ST2361 suggested clonal relation.

Conclusions:

  • MRSP ST2361 presence in humans and hospitals indicates zoonotic and nosocomial transmission risks.
  • Multidrug resistance and virulence genes necessitate enhanced infection control.
  • Urgent need for improved diagnostics and antimicrobial stewardship in healthcare settings.
Keywords:
Antimicrobial resistanceMRSPNosocomial infectionStaphylococcus pseudintermediusZoonotic transmission

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