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Related Concept Videos

MicroRNAs01:22

MicroRNAs

23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
MicroRNAs01:22

MicroRNAs

3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K

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Correction: Marchetti et al. MicroRNA-24-3p Targets Notch and Other Vascular Morphogens to Regulate Post-ischemic Microvascular Responses in Limb Muscles. <i>Int. J. Mol. Sci</i>. 2020, <i>21</i>, 1733.

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Related Experiment Video

Updated: Jan 10, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

323

Exploring microRNAs, One Cell at a Time.

Jessica Kreutz1, Tijana Mitić1, Andrea Caporali1

  • 1Centre for Cardiovascular Science, The Institute for Neuroscience and Cardiovascular Research, The University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

Non-Coding RNA
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

Single-cell sequencing and computational analysis reveal microRNA (miRNA) roles in gene regulation. New tools explore cell-specific miRNA abundance and mechanisms, advancing life sciences and disease understanding.

Keywords:
bioinformaticsmicroRNAsingle-cell sequencingspatial transcriptomics

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Single-cell sequencing and computational analysis have advanced understanding of cellular diversity and gene expression.
  • High-throughput omics technologies generate vast biological data, but single-cell gene regulation, particularly via microRNAs (miRNAs), remains underexplored.
  • MicroRNAs are key gene expression regulators impacting cellular functions in health and disease.

Purpose of the Study:

  • To present experimental tools and computational methods for analyzing cell-specific miRNA abundance.
  • To investigate the mechanisms of microRNA-mediated gene regulation at the single-cell level.
  • To enhance the understanding of miRNA biology and its broader implications for life sciences and diseases.

Main Methods:

  • Single-cell gene expression profiling.
  • Bioinformatic analysis of omics data.
  • Development of experimental and computational tools for miRNA analysis.

Main Results:

  • Uncovered previously hidden miRNA profiles through comprehensive studies.
  • Enabled detailed analysis of cell-specific miRNA abundance.
  • Provided insights into miRNA regulatory mechanisms at the single-cell level.

Conclusions:

  • The developed approaches are expected to reveal the complex nature of miRNA biology.
  • These methods will enhance the understanding of gene regulation at the single-cell level.
  • This work broadly advances life sciences and disease research through improved miRNA analysis.