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Related Experiment Video

Updated: Jun 24, 2026

A Protocol of Manual Tests to Measure Sensation and Pain in Humans

A Protocol of Manual Tests to Measure Sensation and Pain in Humans

Published on: December 19, 2016

Test-Retest Reliability and Sex-Dependent Responses for Physiological and Perceptual Variables at Sub-Maximal

Erik R Snell¹, Pasquale J Succi², Clara J Mitchinson¹

¹Department of Kinesiology and Health Promotion, University of Kentucky, Lexington, KY 40506, USA.

Journal of Functional Morphology and Kinesiology

|November 24, 2025

Summary

The gas exchange threshold (GET) and respiratory compensation point (RCP) show excellent reliability for oxygen consumption and power output. However, heart rate and perceived exertion reliability varied, highlighting sex-specific responses in exercise physiology.

Keywords:

cardiorespiratory fitness exercise prescription fatigue reliability

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Area of Science:

Exercise Physiology
Sports Science
Human Performance

Background:

Fatigue thresholds like the gas exchange threshold (GET) and respiratory compensation point (RCP) are key physiological markers.
Understanding their reliability and sex-specific responses is crucial for exercise science.

Purpose of the Study:

To investigate the reliability of GET and RCP across different expressions (absolute, relative, normalized) of V˙O₂, PO, HR, and RPE.
To examine sex-dependent differences in these physiological responses.

Main Methods:

A test-retest graded exercise test (GXT) protocol was used on healthy males and females.
Linear regression identified PO, HR, and RPE at GET and RCP.
Mixed-model ANOVA and reliability analyses (ICC, MD, SEM, CV) were performed.

Main Results:

Excellent relative reliability was found for absolute and relative V˙O₂ and PO at GET and RCP.
Good to poor relative reliability was observed for HR and RPE, with acceptable absolute reliability.
Males had higher absolute PO and V˙O₂; females showed higher normalized RCP, HR, and RPE at RCP.

Conclusions:

While HR and RPE show variable relative reliability, they possess acceptable absolute reliability for exercise monitoring.
Current exercise prescription may overlook distinct sex-dependent perceptual and cardiovascular responses at GET and RCP.