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Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
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Growth hormone induces GATA3 gene expression via STAT5B
Mana Mitsutani1, Hiromi Hano1, Mei Yokoyama1
1Medicine & Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Growth Factors (Chur, Switzerland)
|November 24, 2025
Summary
Growth hormone (GH) stimulates GATA3 gene expression through the GHR/JAK/STAT5 pathway. This mechanism regulates adipocyte proliferation and differentiation, highlighting GH
Area of Science:
- Molecular Biology
- Cell Biology
- Endocrinology
Background:
- GATA proteins (GATA2, GATA3) are implicated in adipogenesis.
- Extracellular signals regulating GATA proteins in adipogenesis are not fully understood.
Purpose of the Study:
- To investigate if growth hormone (GH) acts as an extracellular ligand for GATA proteins.
- To examine the role of GH in GATA3 expression within adipocytes.
Main Methods:
- Investigated GH's effect on GATA3 expression.
- Analyzed STAT5B activation and its role in GATA3 transcription.
- Identified STAT5B binding sites in the GATA3 promoter region.
Main Results:
- GH treatment increased GATA3 expression transcriptionally.
- STAT5B activation was essential for GH-induced GATA3 expression.
- Functional STAT5B binding sites were identified at -117 bp in the GATA3 promoter.
Conclusions:
- GH induces GATA3 gene expression via the GHR/JAK/STAT5 pathway.
- This pathway regulates adipocyte proliferation and differentiation.
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