Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

3.6K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para...
3.6K
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH301:11

ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3

7.2K
All ortho–para directors, excluding halogens, are activating groups. These groups donate electrons to the ring, making the ring carbons electron-rich. Consequently, the reactivity of the aromatic ring towards electrophilic substitution increases. For instance, the nitration of anisole is about 10,000 times faster than the nitration of benzene. The electron-donating effect of the methoxy group in anisole activates the ortho and para positions on the ring and stabilizes the corresponding...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Buried-interface stabilization for efficient and bright blue perovskite LEDs.

Nature communications·2026
Same author

Activated immune cells present a lung interstitial-to-airway/alveolar cross-compartment sharing pattern in severe pneumonia.

Clinical and translational medicine·2026
Same author

Impacts of climate change on the geographical distribution of rare and endangered Cyatheaceae in China: a MaxEnt model-based prediction.

Scientific reports·2026
Same author

Functional RNA splitting drove the evolutionary emergence of type V CRISPR-Cas systems from transposons.

Cell·2026
Same author

Hierarchically Targeted Cu-Doped Carbon Dot/Kynurenic Acid@RM Platform for Restoring Mitochondrial Metabolism and Modulating Inflammation in Acute Pancreatitis.

ACS nano·2026
Same author

An explainable dual-attention transformer for predicting the sociocultural impact of global sports events.

Scientific reports·2026

Related Experiment Video

Updated: Jan 10, 2026

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
11:02

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

8.1K

An Orthogonally Functionalizable Tetrazine Building Block for Dual-Conjugated Bioorthogonal Probes.

Bing-Feng Wang1, Ke Chen1,2, Xirui Liu1

  • 1Department of Radiology, Huaxi MR Research Center (HMRRC), Institute of Radiology and Medical Imaging, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.

Organic Letters
|November 24, 2025
PubMed
Summary

Researchers developed a new tetrazine building block for creating versatile bioorthogonal tools. This scaffold enables modular construction of dual-conjugated probes that form new fluorophores in situ, aiding theranostic agent design.

More Related Videos

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
10:47

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

9.2K
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
10:54

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Published on: February 4, 2017

8.5K

Related Experiment Videos

Last Updated: Jan 10, 2026

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
11:02

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

8.1K
Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
10:47

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

9.2K
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
10:54

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry

Published on: February 4, 2017

8.5K

Area of Science:

  • Chemical Biology
  • Organic Chemistry
  • Biomedical Research

Background:

  • Bioorthogonal chemistry is crucial for advancing biomedical research.
  • Developing versatile tools is key to expanding the applications of bioorthogonal chemistry.

Purpose of the Study:

  • To create a gram-scale accessible building block for multifunctional bioorthogonal tools.
  • To enable modular construction of dual-conjugated tetrazine probes with diverse functionalities.

Main Methods:

  • Synthesis of a novel tetrazine building block: 3-(mesyloxyethyl)-6-(diethylphosphonomethyl)-tetrazine.
  • Modular assembly of dual-conjugated tetrazine probes.
  • Characterization of the spectral properties of bioorthogonal adducts.

Main Results:

  • The developed building block features two orthogonal reactive handles for modular synthesis.
  • Dual-conjugated tetrazine probes were successfully constructed.
  • The dual-conjugated architecture induced bathochromic emission in bioorthogonal adducts, enabling in situ fluorophore formation.

Conclusions:

  • The novel tetrazine scaffold provides a versatile platform for designing advanced bioorthogonal probes.
  • The ability to form fluorophores in situ offers a promising approach for developing theranostic agents.
  • This work expands the toolkit for chemical biology and biomedical imaging.