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Isolation of a ribonucleoprotein structure from oncornaviruses
Journal of Virology
|December 1, 1973
Summary
Detergent treatment isolates a complex from avian and mammalian oncornaviruses. This complex contains viral RNA, a key arginine-rich protein, and reverse transcriptase activity.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Avian and mammalian oncornaviruses are retroviruses.
- These viruses possess unique structural components and enzymatic activities.
Purpose of the Study:
- To characterize the structure released from oncornaviruses upon detergent treatment.
- To identify the components associated with viral RNA.
Main Methods:
- Oncornaviruses (avian and mammalian) were subjected to detergent treatment.
- The released structures were analyzed for their composition and enzymatic activity.
Main Results:
- Detergent treatment successfully released a specific structure from both avian and mammalian oncornaviruses.
- This structure was found to contain viral RNA.
- A major arginine-rich protein and reverse transcriptase activity were also identified within the released structure.
Conclusions:
- A conserved structural complex, comprising viral RNA, an arginine-rich protein, and reverse transcriptase, is released from oncornaviruses by detergents.
- This finding provides insights into the fundamental organization of these viruses.