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Updated: Jan 10, 2026
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Sampling and Identification of Microplastics in Groundwater
Published on: November 7, 2025
879
Sampling and Identification of Microplastics in Groundwater
Manca Kovač Viršek1, Tine Bizjak2, Nejc Bizjak3
1National Institute of Biology; Geological Survey of Slovenia, Groundwater - Hydrogeology; Institute for Water of the Republic of Slovenia, Sector for Marine Waters; manca.kovac.virsek@nib.si.
Journal of Visualized Experiments : Jove
|November 24, 2025
Summary
This study details a new protocol for sampling and analyzing microplastics in groundwater. It aims to standardize methods for monitoring these pollutants in water sources.
Area of Science:
- Environmental Science
- Analytical Chemistry
Background:
- Microplastic pollution in groundwater is underreported.
- Standardized methods for groundwater microplastic analysis are lacking.
Purpose of the Study:
- To present a comprehensive protocol for groundwater microplastic sampling, separation, and analysis.
- To support the development of synchronized monitoring methodologies for microplastics in water.
Main Methods:
- Detailed description of a filtration sampling system for boreholes.
- Laboratory analysis using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and micro-FTIR spectroscopy.
- Characterization of microplastics by size, shape, color, transparency, and chemical structure.
Main Results:
- A complete protocol for microplastic analysis in groundwater is provided.
- Consideration of factors influencing results and contamination prevention is included.
- Methodology aligns with EU directives for water quality monitoring.
Conclusions:
- The protocol offers a standardized approach to groundwater microplastic assessment.
- This resource, with video guidance, will aid global researchers in microplastic monitoring.
- Ensures consistent and reliable data for environmental and health assessments.
