Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

12.9K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
12.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

6.8K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
6.8K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immediate extubation post-surgery in cardiac patients: a retrospective single-center case series and literature review.

Journal of cardiothoracic surgery·2026
Same author

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same author

Electrical stimulation improves arteriogenic erectile dysfunction by modulating CYLD-mediated macrophage-smooth muscle cell crosstalk.

International immunopharmacology·2026
Same author

Tumor-Derived LAMB3 Drives Immunosuppressive LRRC15<sup>+</sup> Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

DRIFT-EM enables direct wafer retrieval of ultrathin serial sections for large-volume electron microscopy.

Cell reports methods·2026
Same author

Overt physiological responses and atypical behavior evoked in adult zebrafish in the novel tank test by two acute 'survival' stressors.

Behavioural processes·2026
Same journal

Tau protein as a regulator of mitochondrial function and dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A scalable, dividing cell model for the robust propagation and quantification of human sporadic Creutzfeldt-Jakob disease prions.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Epigenetic regulation of mesenchymal BMP signaling directs postnatal organ innervation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Single-shot wide-field biochemical imaging at 1 kHz frame rate.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Morphogenesis and topological evolution of a frustrated nematic liquid crystal under confinement.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

B cell-intrinsic CXCR3 drives efficient generation of ectopic pulmonary germinal center responses to influenza A virus infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
08:47

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation

Published on: January 12, 2024

2.3K

Photoemission electron microscopy for connectomics.

Gregg Wildenberg1,2, Kevin M Boergens3, Lola Lambert1

  • 1Department of Neurobiology, University of Chicago, Chicago, IL 60637.

Proceedings of the National Academy of Sciences of the United States of America
|November 24, 2025
PubMed
Summary
This summary is machine-generated.

Photoemission electron microscopy (PEEM) provides a new method for large-volume connectomics. This technique achieves high-resolution imaging of brain tissue, offering a scalable and cost-effective alternative to existing electron microscopy methods.

Keywords:
Photoemission Electron Microscopy (PEEM)brain mappingconnectomics

More Related Videos

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

13.8K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.1K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
08:47

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation

Published on: January 12, 2024

2.3K
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

13.8K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.1K

Area of Science:

  • Neuroscience
  • Microscopy
  • Connectomics

Background:

  • Large-volume connectomics is crucial for understanding neural circuits.
  • Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are current modalities.
  • A need exists for scalable and cost-effective connectomic imaging techniques.

Purpose of the Study:

  • To evaluate Photoemission electron microscopy (PEEM) as a third modality for large-volume connectomics.
  • To demonstrate PEEM's capability for synaptic resolution imaging.
  • To assess PEEM's potential for high-throughput data acquisition.

Main Methods:

  • Imaging osmium-stained, ultrathin brain sections on gold-coated silicon using commercial PEEMs.
  • Utilizing ultraviolet laser illumination for gigavoxel-per-second acquisition rates.
  • Comparing PEEM performance with established TEM and SEM techniques.

Main Results:

  • Synaptic resolution was achieved using PEEM on brain sections.
  • Gigavoxel-per-second acquisition rates were demonstrated with ultraviolet laser illumination.
  • No thermal damage was observed at high acquisition rates.

Conclusions:

  • PEEM is a viable third modality for large-volume connectomics.
  • PEEM combines parallel detection (TEM-like) with solid supports (SEM-compatible).
  • PEEM presents a potentially scalable and cost-effective approach for mapping neural connectomes.