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Published on: April 23, 2012
Antifungal ergostane-type steroids from endophytic fungus Xylaria sp. VDL4
Jiao Yao1, Lin-Hong Lu1, Sheng-Yun Wei1
1Key Laboratory of State Forestry Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China.
Abstract:
Three ergostane-type steroids, including one rare C-29 methylated xylarstane A (1) and one C22,23-epoxied xylarstane B (2), along with one reported compound 3, were isolated from the endophytic fungus Xylaria sp. VDL4 harbored within the medicinal plant Vaccinium dunalianum. The structures of novel compounds were elucidated through comprehensive spectroscopic analysis, DP4+-validated theoretical 13C nuclear magnetic resonance (NMR) calculations, and electronic circular dichroism (ECD) calculations. The antifungal activities of the isolates against four phytopathogens were assessed in vitro. Compounds 1 and 3 exerted significant inhibition against Alternaria solani and Botrytis cinerea respectively, both with minimal inhibitory concentration (MIC) of 12.5 μg/mL, comparing with the positive control (Carbendazim and Thiabendazole, MICs = 12.5-25.0 μg/mL).
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Fungal Group Zygomycota
Fungal Phylum Basidiomycota
Fungal Phylum Microsporidia
Fungal Phylum Ascomycota

