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Updated: Jan 10, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
Advances and prospects of large DNA fragment editing in plants
Yidi Zhao1, Yingbo Liang2, Zhongfu Ni1
1Frontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, China Agricultural University, Beijing, China.
Abstract:
Structural variations drive plant genome evolution and shape agronomic traits. Manipulating structural variations has great potential to improve complex plant traits and enhance agricultural sustainability. Genome editing technologies have evolved from gene knockouts and base editing to the modification of short DNA fragments, and are now advancing towards the precise manipulation of large DNA fragments. This advancement facilitates targeted, large-scale genomic changes such as deletions, insertions, replacements, inversions, translocations and duplications. In this Review, we summarize recent advances in developing technologies for large DNA fragment editing and highlight their key applications in plants as well as their potential to accelerate crop improvement. Finally, we discuss the current challenges and future prospects for these technologies in plant science.
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